Anticorps Recombinant de lapin anti-RHOA

• Phare
• Validé par KD/KO

RHOA Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240880E5

N° de cat : 82760-2-PBS

Synonymes

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

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Galerie de données de validation

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  WB analysis using 82760-2-RR (same clone as 82760-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82760-2-RR (same clone as 82760-2-PBS)

  WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82760-2-RR (same clone as 82760-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82760-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

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Informations sur le produit

82760-2-PBS cible RHOA dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RHOA Protéine recombinante Ag1141
• Nom complet: ras homolog gene family, member A
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005976
• Symbole du gène: RHOA
• Identification du gène (NCBI): 387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

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