Anticorps Recombinant de lapin anti-RHOA

• Phare
• Validé par KD/KO

RHOA Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240880E5

N° de cat : 82760-2-RR

Synonymes

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

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Galerie de données de validation

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  WB analysis using 82760-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82760-2-RR

  WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells.

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  IF Staining of HeLa using 82760-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82760-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HEK-293, cellules HeLa, cellules HL-60, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82760-2-RR cible RHOA dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RHOA Protéine recombinante Ag1141
• Nom complet: ras homolog gene family, member A
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005976
• Symbole du gène: RHOA
• Identification du gène (NCBI): 387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

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