Anticorps Recombinant de lapin anti-RHOB

RHOB Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251662A8

N° de cat : 86663-3-RR

Synonymes

ARH6, ARHB, h6, Rho cDNA clone 6, Rho-related GTP-binding protein RhoB

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Galerie de données de validation

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  WB analysis using 86663-3-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86663-3-RR (RHOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86663-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86663-3-RR against Human RHOB were performed. The affinity constant is 2.59 nM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86663-3-RR cible RHOB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RHOB Protéine recombinante Ag5593
• Nom complet: ras homolog gene family, member B
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-26 kDa
• Numéro d’acquisition GenBank: BC066954
• Symbole du gène: RHOB
• Identification du gène (NCBI): 388
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rho-related GTP-binding protein RhoB (RHOB) is also named ARH6, ARHB and Rho cDNA clone 6 (h6). RhoB was the first member of the Rho family to be implicated in endosomal trafficking. One study showed that RhoB localizes and activates its downstream target, serine/threonine kinase (PRK1), on endosomes, and acts through this signaling pathway to disrupt the trafficking of internalized EGF receptor from endosomes to a prelysosomal compartment (PMID: 29385717). RhoB is known to be part of the immediate early genetic response to epidermal growth factor, transforming growth factor β, Src activation, or genotoxic stress (PMID:9545335, PMID: 10679283). RhoB was shown to have potential implications for EGF signaling by targeting the activated EGF receptors to the lysosome, which represents an "off-switch" for mitogenic signals (PMID: 1050858). RhoB was also demonstrated to exert a negative regulatory influence on TGF-β-induced transcriptional activation (PMID:9545335). The activity of the RhoB promoter was stimulated by genotoxic treatments indicating its role in the cellular response to DNA damage (PMID:9388198).

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