Anticorps Recombinant de lapin anti-RHOBTB1

RHOBTB1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240253F10

N° de cat : 83342-1-RR

Synonymes

KIAA0740, RHOBTB1

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Galerie de données de validation

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  WB analysis using 83342-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83342-1-RR (RHOBTB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83342-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOBTB1 antibody (83342-1-RR, Clone: 240253F10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83342-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83342-1-RR against Human RHOBTB1 were performed. The affinity constant is 0.502 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HT-29, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83342-1-RR cible RHOBTB1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RHOBTB1 Protéine recombinante Ag3365
• Nom complet: Rho-related BTB domain containing 1
• Masse moléculaire calculée: 696 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC032848
• Symbole du gène: RHOBTB1
• Identification du gène (NCBI): 9886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920).

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