Anticorps Monoclonal anti-RHOC

RHOC Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G1C1

N° de cat : 67542-1-PBS

Synonymes

ARH9, ARHC, 1G1C1, H9, Rho cDNA clone 9

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Galerie de données de validation

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  WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67542-1-Ig (same clone as 67542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67542-1-Ig (same clone as 67542-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67542-1-Ig (same clone as 67542-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67542-1-Ig (same clone as 67542-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RHOC Monoclonal antibody (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67542-1-PBS cible RHOC dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RHOC Protéine recombinante Ag14633
• Nom complet: ras homolog gene family, member C
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC007245
• Symbole du gène: RHOC
• Identification du gène (NCBI): 389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

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