Anticorps Polyclonal de lapin anti-RHOF

RHOF Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 12290-1-AP

Synonymes

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

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Galerie de données de validation

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  WB analysis using 12290-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12290-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12290-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12290-1-AP

  IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12290-1-AP cible RHOF dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHOF Protéine recombinante Ag2943
• Nom complet: ras homolog gene family, member F (in filopodia)
• Masse moléculaire calculée: 211 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC018208
• Symbole du gène: RHOF
• Identification du gène (NCBI): 54509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression; Authors - Michiru Nishita
human	WB	Int J Mol Sci; An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer; Authors - Yongcheng Su
human,mouse	WB,IHC,IF	iScience; RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance; Authors - Xin Wen ; KD Validated
human	IHC	Virchows Arch; High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma; Authors - Xinyi Zhu
human	WB	EPMA J; Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies; Authors - Jinjin Zhang

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