Anticorps Monoclonal anti-RIAM

RIAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F8A2

N° de cat : 67143-1-PBS

Synonymes

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

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Galerie de données de validation

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  WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67143-1-Ig (same clone as 67143-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67143-1-Ig (same clone as 67143-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67143-1-Ig (same clone as 67143-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67143-1-PBS cible RIAM dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RIAM Protéine recombinante Ag4602
• Nom complet: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
• Masse moléculaire calculée: 666 aa, 73 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC035636
• Symbole du gène: RIAM
• Identification du gène (NCBI): 54518
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.

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