Anticorps Polyclonal de lapin anti-RICTOR

• Phare
• Validé par KD/KO

RICTOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(25)

Avis (1)

N° de cat : 27248-1-AP

Synonymes

Rapamycin-insensitive companion of mTOR, KIAA1999, hAVO3, AVO3 homolog

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Galerie de données de validation

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  WB analysis using 27248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27248-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 27248-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27248-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27248-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 27248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (27248-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 27248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RICTOR antibody (27248-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 27248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27248-1-AP (RICTOR antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

27248-1-AP cible RICTOR dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RICTOR Protéine recombinante Ag25649
• Nom complet: rapamycin-insensitive companion of mTOR
• Masse moléculaire calculée: 192 kDa
• Poids moléculaire observé: 192 kDa
• Numéro d’acquisition GenBank: BC029608
• Symbole du gène: RICTOR
• Identification du gène (NCBI): 253260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Aging (Albany NY); Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts; Authors - Shi-Yu Wang
mouse	WB,IF	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
mouse	IF	J Cell Physiol; Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes; Authors - Rui Hua
human	WB	J Ethnopharmacol; Diterpenoid tanshinones inhibit gastric cancer angiogenesis through the PI3K/Akt/mTOR signaling pathway; Authors - Jie-Ru Yu
human	WB	Cell Commun Signal; Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.; Authors - Norihiko Sasaki

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-12-2019)

The antibody recognizes RICTOR which is around ~200kD. It is the top most band. The first two lanes were cell lysates and show that the antibody recognizes other proteins non-specifically. The last lane contained eluted proteins that are presumably proximal to the cell membrane. RICTOR shows up there cleanly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

RICTOR Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:27248-1-AP)

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