Anticorps Polyclonal de lapin anti-RIG-1/DDX58

• Phare
• Validé par KD/KO

RIG-1/DDX58 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20566-1-PBS

Synonymes

DDX58, RIG-I, Retinoic acid-inducible gene 1 protein, EC:3.6.4.13, DEAD box protein 58

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Galerie de données de validation

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  WB analysis using 20566-1-AP (same clone as 20566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20566-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20566-1-AP (same clone as 20566-1-PBS)

  WB result of RIG-1/DDX58 antibody (20566-1-AP; 1:2000; room temperature for 1.5 hours) with wild-type and RIG-1/DDX58 knockout A431 cells. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 20566-1-AP (same clone as 20566-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20566-1-AP (same clone as 20566-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

  IP result of anti-RIG-1/DDX58 (IP:20566-1-AP, 4ug; Detection:20566-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 20566-1-AP (same clone as 20566-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using RIG-1/DDX58 antibody (20566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20566-1-PBS cible RIG-1/DDX58 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Masse moléculaire calculée: 106 kDa
• Poids moléculaire observé: 101~106 kDa
• Numéro d’acquisition GenBank: NM_014314
• Symbole du gène: RIG-1/DDX58
• Identification du gène (NCBI): 23586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

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