Anticorps Recombinant de lapin anti-RIG-1/DDX58

RIG-1/DDX58 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242424E7

N° de cat : 84861-5-RR

Synonymes

DDX58, RIG-I, 242424E7, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58

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Galerie de données de validation

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  WB analysis of A431 using 84861-5-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84861-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84861-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84861-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84861-5-RR (RIG-1/DDX58 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 84861-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RIG-1/DDX58 antibody (84861-5-RR, Clone: 242424E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84861-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84861-5-RR against Human RIG-1/DDX58 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7, cellules THP-1
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84861-5-RR cible RIG-1/DDX58 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RIG-1/DDX58 Protéine recombinante Ag18585
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Masse moléculaire calculée: 925 aa, 106 kDa
• Poids moléculaire observé: 101~106 kDa
• Numéro d’acquisition GenBank: BC132786
• Symbole du gène: RIG-1/DDX58
• Identification du gène (NCBI): 23586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

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