Anticorps Recombinant de lapin anti-RILP

• Phare
• Validé par KD/KO

RILP Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230208G2

N° de cat : 82996-4-PBS

Synonymes

230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein

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Galerie de données de validation

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  WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82996-4-RR (same clone as 82996-4-PBS)

  WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82996-4-RR (same clone as 82996-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RILP Recombinant antibody (82996-4-RR, Clone:230208G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00025-1

  Cytometric bead array standard curve of MP00025-1, RILP Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82996-4-PBS. Detection antibody: 82996-3-PBS. Standard: Ag4472. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 82996-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82996-4-PBS cible RILP dans les applications de WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RILP Protéine recombinante Ag4472
• Nom complet: Rab interacting lysosomal protein
• Masse moléculaire calculée: 401 aa, 41 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC031621
• Symbole du gène: RILP
• Identification du gène (NCBI): 83547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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