Anticorps Polyclonal de lapin anti-RIOK3

• Phare
• Validé par KD/KO

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13593-1-PBS

Synonymes

sudD homolog, SUDD, Serine/threonine-protein kinase RIO3, RIO kinase 3 (yeast), RIO kinase 3

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Galerie de données de validation

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  WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  WB analysis using 13593-1-AP (same clone as 13593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13593-1-AP (same clone as 13593-1-PBS)

  IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13593-1-AP (same clone as 13593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13593-1-AP (same clone as 13593-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13593-1-AP (same clone as 13593-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13593-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13593-1-PBS cible RIOK3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RIOK3 Protéine recombinante Ag4497
• Nom complet: RIO kinase 3 (yeast)
• Masse moléculaire calculée: 516 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC039729
• Symbole du gène: RIOK3
• Identification du gène (NCBI): 8780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.

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