Anticorps Monoclonal anti-Phospho-RIPK1 (Ser161)

Phospho-RIPK1 (Ser161) Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1B2G1

N° de cat : 66854-1-Ig

Synonymes

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

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Galerie de données de validation

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  WB analysis using 66854-1-Ig

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 66854-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was untreated (left) or treated with Lambda Protein Phosphatase (λ-PPase, 500U, right) at 37℃ for 1 hours before blocking.

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  IHC staining of human small intestine using 66854-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 66854-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66854-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66854-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 66854-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293T using 66854-1-Ig

  1X10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Anti-Human Phospho-RIPK1 (Ser161) (66854-1-Ig, Clone:1B2G1) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293T traitées à la calyculine A, cellules Raji, cellules THP-1
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,
• Résultats positifs en cytométrie: cellules HEK-293T traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000- 1:20000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

66854-1-Ig cible Phospho-RIPK1 (Ser161) dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 74 kDa, 45 kDa
• Numéro d’acquisition GenBank: NM_003804
• Symbole du gène: RIPK1
• Identification du gène (NCBI): 8737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

Publications

Species	Application	Title
human	WB	Inflammation; Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected Macrophages; Authors - Jingjing Shen
human	WB	J Cell Mol Med; LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.; Authors - Chia-Kang Ho
human	WB	Front Genet; Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.; Authors - Yongchang Yang
mouse	WB,IHC	Am J Physiol Renal Physiol; Myo-inositol oxygenase overexpression exacerbates cadmium-induced kidney injury via oxidant stress and necroptosis.; Authors - Xiaoping Zheng
human	WB	Eur J Pharmacol; A natural chalcone cardamonin inhibits necroptosis and ameliorates dextran sulfate sodium (DSS)-induced colitis by targeting RIPK1/3 kinases; Authors - Xiaofei Shen
human	WB	Front Oncol; Identification of necroptosis-related genes for predicting prognosis and exploring immune infiltration landscape in colon adenocarcinoma; Authors - Ye Wang