Anticorps Polyclonal de lapin anti-Phospho-RIPK1 (Ser166)

Phospho-RIPK1 (Ser166) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 28252-1-AP

Synonymes

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser166), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1

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Galerie de données de validation

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  WB analysis using 28252-1-AP

  Non-treated HT-29 and TNF alpha (HZ-1014) treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28252-1-AP (Phospho-RIPK1 (Ser166) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules HT-29 traitées à l'IFN alpha,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

28252-1-AP cible Phospho-RIPK1 (Ser166) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_003804
• Symbole du gène: RIPK1
• Identification du gène (NCBI): 8737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; Necroptosis enhances 'don't eat me' signal and induces macrophage extracellular traps to promote pancreatic cancer liver metastasis; Authors - Cheng-Yu Liao
human	WB	Int Immunopharmacol; EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis; Authors - Huiling Zhang
human	WB	Cell Death Discov; LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening.; Authors - Lulu Wo
human	WB	Inflamm Bowel Dis; Phosphoproteomics Reveals Novel Insights into the Pathogenesis and Identifies New Therapeutic Kinase Targets of Ulcerative Colitis; Authors - Sifan Tao
human,mouse	WB,IHC,IF	iScience; Inhibition of RIPK1 alleviating vascular smooth muscle cells osteogenic transdifferentiation via Runx2; Authors - Yue Li
mouse	IHC	World J Gastroenterol; OSW-1 triggers necroptosis in colorectal cancer cells through the RIPK1/RIPK3/MLKL signaling pathway facilitated by the RIPK1-p62/SQSTM1 complex; Authors - Nan Wang

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