Anticorps Polyclonal de lapin anti-RIPK2

• Phare
• Validé par KD/KO

RIPK2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 15366-1-AP

Synonymes

CARD3, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARDIAK, CCK

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Galerie de données de validation

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  WB analysis using 15366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15366-1-AP (RIPK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15366-1-AP

  WB result of RIPK2 antibody (15366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIPK2 transfected HeLa cells.

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  WB analysis of THP-1 using 15366-1-AP

  WB result of RIPK2 antibody (15366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RIPK2 transfected THP-1 cells.

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  IP experiment of HEK-293T using 15366-1-AP

  IP result of anti-RIPK2 (IP:15366-1-AP, 4ug; Detection:15366-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules THP-1
• Résultats positifs en IP: cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15366-1-AP cible RIPK2 dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RIPK2 Protéine recombinante Ag0909
• Nom complet: receptor-interacting serine-threonine kinase 2
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 46-55 kDa, 61-72 kDa
• Numéro d’acquisition GenBank: BC004553
• Symbole du gène: RIPK2
• Identification du gène (NCBI): 8767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	J Neuroinflammation; H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes; Authors - Jing Li
pig	WB	Antioxidants (Basel); M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide; Authors - Menghui Xu
human	WB	Obesity (Silver Spring); Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.; Authors - Yanping Wang
human	WB	J Pharmacol Exp Ther; Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.; Authors - Mohamed Salla ; KD Validated
mouse	WB	Front Cell Infect Microbiol; Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.; Authors - Chun-Xia Shi
mouse	WB,IP	Immunol Lett; A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation.; Authors - Dong-Yi Cao

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