Anticorps Recombinant de lapin anti-RIT1

RIT1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242593E4

N° de cat : 85347-1-PBS

Synonymes

RIBB, Ras like without CAAX protein 1, Ras like without CAAX 1, Ras like protein expressed in many tissues, GTP binding protein Rit1

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Galerie de données de validation

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  WB analysis using 85347-1-RR (same clone as 85347-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 85347-1-RR (same clone as 85347-1-PBS)

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85347-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85347-1-RR against Human RIT1 were performed. The affinity constant is 0.505 nM.

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Informations sur le produit

85347-1-PBS cible RIT1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RIT1 Protéine recombinante Ag25636
• Nom complet: Ras-like without CAAX 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC104186
• Symbole du gène: RIT1
• Identification du gène (NCBI): 6016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).

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