Anticorps Recombinant de lapin anti-RIT1

RIT1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242593E4

N° de cat : 85347-1-RR

Synonymes

RIBB, Ras like without CAAX protein 1, Ras like without CAAX 1, Ras like protein expressed in many tissues, GTP binding protein Rit1

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Galerie de données de validation

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  WB analysis using 85347-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 85347-1-RR

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85347-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85347-1-RR against Human RIT1 were performed. The affinity constant is 0.505 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85347-1-RR cible RIT1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RIT1 Protéine recombinante Ag25636
• Nom complet: Ras-like without CAAX 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC104186
• Symbole du gène: RIT1
• Identification du gène (NCBI): 6016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).

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