Anticorps Polyclonal de lapin anti-RIT2

RIT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12473-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

  IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

  IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12473-1-PBS cible RIT2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RIT2 Protéine recombinante Ag3160
• Nom complet: Ras-like without CAAX 2
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC018060
• Symbole du gène: RIT2
• Identification du gène (NCBI): 6014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).

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