Anticorps Recombinant de lapin anti-RLBP1

RLBP1 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242989D1

N° de cat : 85697-1-PBS

Synonymes

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

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Galerie de données de validation

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  WB analysis using 85697-1-RR (same clone as 85697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  WB analysis of ARPE-19 using 85697-1-RR (same clone as 85697-1-PBS)

  ARPE-19 cells were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (85697-1-RR, Clone: 242989D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85697-1-PBS cible RLBP1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RLBP1 Protéine recombinante Ag7602
• Nom complet: retinaldehyde binding protein 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004199
• Symbole du gène: RLBP1
• Identification du gène (NCBI): 6017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

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