Anticorps Polyclonal de lapin anti-RMND5A

• Phare
• Validé par KD/KO

RMND5A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 17559-1-AP

Synonymes

RMD5, Protein RMD5 homolog A, P44CTLH, EC:2.3.2.27, E3 ubiquitin-protein transferase RMND5A

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Galerie de données de validation

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  WB analysis using 17559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

17559-1-AP cible RMND5A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RMND5A Protéine recombinante Ag11751
• Nom complet: required for meiotic nuclear division 5 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 391 aa, 44 kDa
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: BC047668
• Symbole du gène: RMND5A
• Identification du gène (NCBI): 64795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.; Authors - Chunyuan Tang ; KD Validated
human	WB	Chembiochem; Identifying E3 ligase substrates with quantitative degradation proteomics; Authors - Victoria N Jordan
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

RMND5A Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:17559-1-AP)

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