Anticorps Polyclonal de lapin anti-RMP

• Phare
• Validé par KD/KO

RMP Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11277-1-AP

Synonymes

C19orf2, NNX3, PPP1R19, Protein NNX3, RMP, RPB5 mediating protein, URI

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Galerie de données de validation

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  WB analysis of A549 using 11277-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11277-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11277-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11277-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11277-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11277-1-AP (RMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11277-1-AP

  IP Result of anti-RMP (IP:11277-1-AP, 4ug; Detection:11277-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human lymphoma using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11277-1-AP (RMP antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11277-1-AP cible RMP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RMP Protéine recombinante Ag1789
• Nom complet: chromosome 19 open reading frame 2
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC026184
• Symbole du gène: C19orf2
• Identification du gène (NCBI): 8725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding ; KD Validated
human	WB	Oncogene; RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.; Authors - J Zhang ; KD Validated
human	WB, IHC	Cell Death Dis; URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription.; Authors - J-L Fan ; KD Validated
human	IHC	Oncotarget; RMP predicts survival and adjuvant TACE response in hepatocellular carcinoma.; Authors - Jian Zhang
human	WB,IHC	Cardiovasc Res; Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure.; Authors - Jian Zhang
human	WB,IHC,IF	Oxid Med Cell Longev; Macrophage Rmp Ameliorates Myocardial Infarction by Modulating Macrophage Polarization in Mice; Authors - Jian Zhang