Anticorps Monoclonal anti-RNASEH1

RNASEH1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2C8D3

N° de cat : 68787-3-Ig

Synonymes

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3

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Galerie de données de validation

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  WB analysis using 68787-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68787-3-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: SK-N-SH cells, cellules 4T1, cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68787-3-Ig cible RNASEH1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RNASEH1 Protéine recombinante Ag33948
• Nom complet: ribonuclease H1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002973
• Symbole du gène: RNASEH1
• Identification du gène (NCBI): 246243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

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