Anticorps Recombinant de lapin anti-RNASEH1

• Phare
• Validé par KD/KO

RNASEH1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C12

N° de cat : 82771-1-PBS

Synonymes

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

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Galerie de données de validation

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  WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

  WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

  WB result of RNASEH1 antibody (82771-1-RR; 1:15000; room temperature for 1.5 hours) with wild-type and RNASEH1 knockout HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82771-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82771-1-PBS cible RNASEH1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNASEH1 Protéine recombinante Ag7990
• Nom complet: ribonuclease H1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC002973
• Symbole du gène: RNASEH1
• Identification du gène (NCBI): 246243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

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