Anticorps Recombinant de lapin anti-RNASEH2B

RNASEH2B Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241131F1

N° de cat : 84097-6-RR

Synonymes

ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2

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Galerie de données de validation

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  WB analysis using 84097-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84097-6-RR (RNASEH2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84097-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNASEH2B antibody (84097-6-RR, Clone: 241131F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84097-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human RNASEH2B were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules Jurkat, cellules THP-1, cellules U-937
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84097-6-RR cible RNASEH2B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNASEH2B Protéine recombinante Ag33669
• Nom complet: ribonuclease H2, subunit B
• Masse moléculaire calculée: 331 aa, 37 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC036744
• Symbole du gène: RNASEH2B
• Identification du gène (NCBI): 79621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

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