Anticorps Monoclonal anti-RND3

• Phare
• Validé par KD/KO

RND3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3H8F9

Avis (1)

N° de cat : 66228-1-Ig

Synonymes

ARHE, memB, Protein MemB, Rho family GTPase 3, Rho8, RhoE, RND3

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Galerie de données de validation

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  WB analysis of fetal human brain using 66228-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66228-1-Ig (RND3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

66228-1-Ig cible RND3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RND3 Protéine recombinante Ag18839
• Nom complet: Rho family GTPase 3
• Masse moléculaire calculée: 244 aa, 27 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC012513
• Symbole du gène: RND3
• Identification du gène (NCBI): 390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Downregulated lncRNA HOXA11-AS Affects Trophoblast Cell Proliferation and Migration by Regulating RND3 and HOXA7 Expression in PE.; Authors - Yetao Xu
human	WB	Int J Mol Med; Ingenuity pathway analysis of differentially expressed genes involved in signaling pathways and molecular networks in RhoE gene‑edited cardiomyocytes.; Authors - Zhongming Shao ; KO Validated
human	WB	Virology; Transcriptomic analysis reveals crucial regulatory roles of immediate-early response genes and related signaling pathways in coronavirus infectious bronchitis virus infection; Authors - Li Xia Yuan
human	IF	bioRxiv; The Hexosamine Biosynthetic Pathway alters the cytoskeleton to modulate cell proliferation and migration in metastatic prostate cancer; Authors - Rajina Shakya

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (12-10-2020)

This antibody is very good quality and can detect RND3 in both U937 and PBMC cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U937 & PBMC

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