Anticorps Polyclonal de lapin anti-RNF10

• Phare
• Validé par KD/KO

RNF10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16936-1-AP

Synonymes

RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10

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Galerie de données de validation

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16936-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 16936-1-AP

  IP result of anti-RNF10 (IP:16936-1-AP, 4ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 1480 ug.

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16936-1-AP (RNF10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16936-1-AP (RNF10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16936-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF10 antibody (16936-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16936-1-AP cible RNF10 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF10 Protéine recombinante Ag10433
• Nom complet: ring finger protein 10
• Masse moléculaire calculée: 811 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC016622
• Symbole du gène: RNF10
• Identification du gène (NCBI): 9921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

Publications

Species	Application	Title
human	WB,IF	Elife; Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.; Authors - Margarita C Dinamarca ; KD Validated
human	WB	Cell Rep; The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.; Authors - Aitor Garzia ; KO Validated
mouse,human	WB	Nat Cell Biol; Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition; Authors - Weiwei Cui
mouse		J Clin Invest; RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication; Authors - Joonyoung Her
human	WB	Nat Commun; E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance; Authors - Janina A Lehmann
human	WB	Mol Cell; The integrated stress response regulates 18S nonfunctional rRNA decay in mammals; Authors - Aaztli R Coria ; KO Validated

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