Anticorps Recombinant de lapin anti-RNF10

RNF10 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250438C11

N° de cat : 86019-1-PBS

Synonymes

E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10

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Galerie de données de validation

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  WB analysis of Jurkat using 86019-1-RR (same clone as 86019-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 86019-1-RR (same clone as 86019-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 86019-1-RR (same clone as 86019-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  IF Staining of K562 using 86019-1-RR (same clone as 86019-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K562 using RNF10 antibody (86019-1-RR, Clone: 250438C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86019-1-PBS cible RNF10 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF10 Protéine recombinante Ag10433
• Nom complet: ring finger protein 10
• Masse moléculaire calculée: 811 aa, 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC016622
• Symbole du gène: RNF10
• Identification du gène (NCBI): 9921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

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