Anticorps Monoclonal anti-RNF126

• Phare
• Validé par KD/KO

RNF126 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1E1B10

N° de cat : 66647-1-Ig

Synonymes

1E1B10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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Galerie de données de validation

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  WB analysis of Jurkat using 66647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66647-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66647-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 66647-1-Ig

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Monoclonal antibody (66647-1-Ig, Clone:1E1B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HSC-T6, cellules K-562, cellules Ramos, cellules RAW 264.7
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66647-1-Ig cible RNF126 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RNF126 Protéine recombinante Ag13816
• Nom complet: ring finger protein 126
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC001442
• Symbole du gène: RNF126
• Identification du gène (NCBI): 55658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

Publications

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu ; KD Validated
human	WB	Exp Eye Res; Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.; Authors - Jing Zhang
human	WB	Cell Death Dis; ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.; Authors - Shengjun Geng
human	WB	Cell Chem Biol; Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway; Authors - Yali Ci
human	IHC,IF	J Cell Physiol; ZNF263 cooperates with ZNF31 to promote the drug resistance and EMT of pancreatic cancer through transactivating RNF126; Authors - Jiawei Zhang
human	WB	ACS Cent Sci; DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders; Authors - Melissa Lim ; KO Validated

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