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Anticorps Polyclonal de lapin anti-RNF144A

RNF144A Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, Hamster

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26144-1-PBS

Synonymes

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of MDA-MB-231 cells using RNF144A Polyclonal antibody (26144-1-AP)

    WB analysis of MDA-MB-231 using 26144-1-AP (same clone as 26144-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of CHO cells using RNF144A Polyclonal antibody (26144-1-AP)

    IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of CHO cells using RNF144A Polyclonal antibody (26144-1-AP)

    IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3080ug. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

Informations sur le produit

26144-1-PBS cible RNF144A dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, Hamster

Réactivité Humain, Hamster
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RNF144A Protéine recombinante Ag23508
Nom complet ring finger protein 144A
Poids moléculaire observé 33 kDa
Numéro d’acquisition GenBankBC050373
Symbole du gène RNF144A
Identification du gène (NCBI) 9781
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).

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