Anticorps Recombinant de lapin anti-RNF160

• Phare
• Validé par KD/KO

RNF160 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230075H2

N° de cat : 82926-1-PBS

Synonymes

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

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Galerie de données de validation

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  WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82926-1-RR (same clone as 82926-1-PBS)

  WB result of RNF160 antibody (82926-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF160 transfected HepG2 cells. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82926-1-PBS cible RNF160 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF160 Protéine recombinante Ag29467
• Nom complet: ring finger protein 160
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC140790
• Symbole du gène: RNF160
• Identification du gène (NCBI): 26046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

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