Anticorps Polyclonal de lapin anti-RNF167

• Phare
• Validé par KD/KO

RNF167 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 24618-1-AP

Synonymes

E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 24618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 24618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24618-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HaCaT using 24618-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RNF167 antibody (24618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules HeLa, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HaCaT,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

24618-1-AP cible RNF167 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF167 Protéine recombinante Ag18383
• Nom complet: ring finger protein 167
• Masse moléculaire calculée: 350 aa, 38 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC010139
• Symbole du gène: RNF167
• Identification du gène (NCBI): 26001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

Publications

Species	Application	Title
human	WB	Nat Commun; RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.; Authors - Tingting Li ; KD Validated
human	WB	FASEB J; RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation; Authors - Zhenzhen Yan
mouse,human	WB,IP	Nat Commun; RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways; Authors - Miao He ; KD Validated

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