Anticorps Polyclonal de lapin anti-RNF180

• Phare
• Validé par KD/KO

RNF180 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30941-1-PBS

Synonymes

E3 ubiquitin-protein ligase RNF180, EC:2.3.2.27, RINES, RING finger protein 180, RNF 180

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Galerie de données de validation

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  WB analysis using 30941-1-AP (same clone as 30941-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30941-1-AP (RNF180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 30941-1-AP (same clone as 30941-1-PBS)

  WB result of RNF180 antibody (30941-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF180 transfected A549 cells. This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 30941-1-AP (same clone as 30941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30941-1-AP (RNF180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 30941-1-AP (same clone as 30941-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RNF180 antibody (30941-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30941-1-PBS cible RNF180 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF180 Protéine recombinante Ag34138
• Nom complet: ring finger protein 180
• Masse moléculaire calculée: 592 aa, 68 kDa
• Poids moléculaire observé: 70 kDa,48kDa
• Numéro d’acquisition GenBank: BC101277
• Symbole du gène: RNF180
• Identification du gène (NCBI): 285671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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