Anticorps Recombinant de lapin anti-RNF181

RNF181 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243121B5

N° de cat : 85735-2-PBS

Synonymes

E3 ubiquitin-protein ligase RNF181, EC:2.3.2.27, HSPC238, RING finger protein 181, RN181

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Galerie de données de validation

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  WB analysis using 85735-2-RR (same clone as 85735-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85735-2-RR (RNF181 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85735-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85735-2-RR (same clone as 85735-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RNF181 antibody (85735-2-RR, Clone: 243121B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85735-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02066-1

  Cytometric bead array standard curve of MP02066-1, RNF181 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85735-2-PBS. Detection antibody: 85735-4-PBS. Standard: Ag14233. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 85735-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85735-2-RR against Human RNF181 were performed. The affinity constant is 0.138 nM.

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Informations sur le produit

85735-2-PBS cible RNF181 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF181 Protéine recombinante Ag14233
• Nom complet: ring finger protein 181
• Masse moléculaire calculée: 153 aa, 18 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC002803
• Symbole du gène: RNF181
• Identification du gène (NCBI): 51255
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

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