Anticorps Polyclonal de lapin anti-RNF220

RNF220 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32482-1-PBS

Synonymes

RING-type E3 ubiquitin transferase RNF220, RING finger protein 220, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF220, C1orf164

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Galerie de données de validation

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  WB analysis using 32482-1-AP (same clone as 32482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32482-1-AP (RNF220 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32482-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32482-1-PBS cible RNF220 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF220 Protéine recombinante Ag36190
• Nom complet: ring finger protein 220
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC034221
• Symbole du gène: RNF220
• Identification du gène (NCBI): 55182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF220 is an E3 ubiquitin ligase that plays a crucial role in the ubiquitination process, which targets proteins for degradation by the proteasome. Mutations in RNF220 have been linked to laminopathies, which are characterized by leukodystrophy, ataxia, and deafness. Additionally, RNF220 is involved in the progression of certain cancers, such as acute myeloid leukemia (AML) and colorectal cancer. Studies have shown that RNF220 promotes the proliferation of leukemia cells and reduces the degradation of Cyclin D1 protein through USP22. It also enhances canonical Wnt signaling through USP7-mediated deubiquitination of beta-catenin. (PMID: 32896826)

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