Anticorps Polyclonal de lapin anti-RNF31 / HOIP

RNF31 / HOIP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16289-1-AP

Synonymes

HOIP, RNF31, HOIL-1-interacting protein, HOIL 1 interacting protein, FLJ23501

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Galerie de données de validation

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  WB analysis of HeLa using 16289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16289-1-AP (HOIP antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected cell cell using 16289-1-AP

  WB result of RNF31/HOIP antibody (16289-1-AP) by Emmanuel Laplantine (Institute Pasteur,Paris,France).

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  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, Transfected cell cell
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

16289-1-AP cible RNF31 / HOIP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF31 / HOIP Protéine recombinante Ag9440
• Nom complet: ring finger protein 31
• Masse moléculaire calculée: 1072 aa, 120 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC017376
• Symbole du gène: RNF31
• Identification du gène (NCBI): 55072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)

Publications

Species	Application	Title
human	WB	Oncogene; AGO4 suppresses tumor growth by modulating autophagy and apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in a p53-independent manner; Authors - Lin Wang
mouse	WB	J Immunol; HOIL1 Regulates Group 3 Innate Lymphoid Cells in the Colon and Protects against Systemic Dissemination, Colonic Ulceration, and Lethality from Citrobacter rodentium Infection; Authors - Victoria L Hartley
human	WB	Bioorg Med Chem; Biophysical and biological evaluation of optimized stapled peptide inhibitors of the linear ubiquitin chain assembly complex (LUBAC).; Authors - Francisco Aguilar-Alonso
human	WB	BMC Musculoskelet Disord; Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.; Authors - Lin Chen
mouse	WB	Int Immunopharmacol; OTULIN orchestrates NCOA4-FTH1 complex to alleviate APAP-induced hepatocyte Ferroptosis; Authors - Liyuan Gao
human	WB	Arch Oral Biol; A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.; Authors - Shichen Sun

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