Anticorps Recombinant de lapin anti-RNF31 / HOIP

RNF31 / HOIP Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230446H1

N° de cat : 83137-3-PBS

Synonymes

HOIP, RNF31, 230446H1, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31

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Galerie de données de validation

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  IF Staining of HepG2 using 83137-3-RR (same clone as 83137-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 83137-3-RR (same clone as 83137-3-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00285-1

  Cytometric bead array standard curve of MP00285-1, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-1-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

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  Cytometric bead array standard curve of MP00285-2

  Cytometric bead array standard curve of MP00285-2, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-2-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83137-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83137-3-PBS cible RNF31 / HOIP dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF31 / HOIP Protéine recombinante Ag34505
• Nom complet: ring finger protein 31
• Masse moléculaire calculée: 1072 aa, 120 kDa
• Numéro d’acquisition GenBank: BC017376
• Symbole du gène: RNF31
• Identification du gène (NCBI): 55072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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