Anticorps Polyclonal de lapin anti-RNF31 / HOIP

• Phare
• Validé par KD/KO

RNF31 / HOIP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30797-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30797-1-AP (same clone as 30797-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 30797-1-AP (same clone as 30797-1-PBS)

  WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 30797-1-AP (same clone as 30797-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30797-1-PBS cible RNF31 / HOIP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF31 / HOIP Protéine recombinante Ag34505
• Nom complet: ring finger protein 31
• Masse moléculaire calculée: 1072 aa, 120 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC017376
• Symbole du gène: RNF31
• Identification du gène (NCBI): 55072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)

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