Anticorps Polyclonal de lapin anti-RNF38

RNF38 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25132-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25132-1-AP (RNF38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25132-1-AP (RNF38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25132-1-PBS cible RNF38 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF38 Protéine recombinante Ag16054
• Nom complet: ring finger protein 38
• Masse moléculaire calculée: 515 aa, 58 kDa
• Poids moléculaire observé: 43-58 kDa
• Numéro d’acquisition GenBank: BC033786
• Symbole du gène: RNF38
• Identification du gène (NCBI): 152006
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis. RNF38 exists various isoforms and molecular weight of isoforms are 36, 48, 52 and 58 kDa

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