Anticorps Polyclonal de lapin anti-RNF4

• Phare
• Validé par KD/KO

RNF4 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 17810-1-AP

Synonymes

E3 ubiquitin ligase RNF4, Protein SNURF, RES4 26, ring finger protein 4, RNF4, SNURF

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Galerie de données de validation

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  WB analysis of PC-3 using 17810-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 17810-1-AP

  IP Result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:300) with PC-3 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules PC-3,
• Résultats positifs en IP: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

17810-1-AP cible RNF4 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF4 Protéine recombinante Ag12115
• Nom complet: ring finger protein 4
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC031935
• Symbole du gène: RNF4
• Identification du gène (NCBI): 6047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

Publications

Species	Application	Title
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB	Int J Mol Sci; The E3 Ubiquitin-Protein Ligase RNF4 Promotes TNF-α-Induced Cell Death Triggered by RIPK1.; Authors - Tatsuya Shimada
human	WB	ACS Chem Biol; Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications.; Authors - Carl C Ward
human	WB	J Biol Chem; Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control; Authors - Wei Wang ; KD Validated
mouse	WB,CoIP	Theranostics; CSF2RB overexpression promotes the protective effects of mesenchymal stromal cells against ischemic heart injury; Authors - Tingting Qi ; KD Validated
human	WB	Exp Cell Res; RNF4 promotes tumorigenesis, therapy resistance of cholangiocarcinoma and affects cell cycle by regulating the ubiquitination degradation of p27kip1 in the nucleus; Authors - Jie Huang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 cells