Anticorps Polyclonal de lapin anti-RNF4

• Phare
• Validé par KD/KO

RNF4 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17810-1-PBS

Synonymes

RES4-26, RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4

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Galerie de données de validation

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  WB analysis of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

  IP result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:1500) with PC-3 cells lysate 1800 ug. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17810-1-PBS cible RNF4 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF4 Protéine recombinante Ag12115
• Nom complet: ring finger protein 4
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC031935
• Symbole du gène: RNF4
• Identification du gène (NCBI): 6047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

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