Anticorps Recombinant de lapin anti-RNF4

RNF4 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242197A10

N° de cat : 84770-1-RR

Synonymes

242197A10, E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF

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Galerie de données de validation

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  IF Staining of HepG2 using 84770-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF4 antibody (84770-1-RR, Clone: 242197A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of MCF-7 using 84770-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RNF4 Recombinant antibody (84770-1-RR, Clone:242197A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84770-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84770-1-RR against Human RNF4 were performed. The affinity constant is 17.3 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84770-1-RR cible RNF4 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF4 Protéine recombinante Ag12115
• Nom complet: ring finger protein 4
• Masse moléculaire calculée: 190 aa, 21 kDa
• Numéro d’acquisition GenBank: BC031935
• Symbole du gène: RNF4
• Identification du gène (NCBI): 6047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220].

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