Anticorps Polyclonal de lapin anti-RNF40

RNF40 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 15621-1-AP

Synonymes

BRE1 B, BRE1B, KIAA0661, RBP95, ring finger protein 40, RNF40, STARING

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Galerie de données de validation

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  WB analysis of human brain using 15621-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15621-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15621-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15621-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15621-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15621-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu hépatique de souris, tissu pulmonaire de souris, tissu splénique de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15621-1-AP cible RNF40 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF40 Protéine recombinante Ag8047
• Nom complet: ring finger protein 40
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC006133
• Symbole du gène: RNF40
• Identification du gène (NCBI): 9810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (03-11-2022)

This antibody works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Colon cancer cells