Anticorps Polyclonal de lapin anti-RNF40

RNF40 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (2)

N° de cat : 15621-1-AP

Synonymes

EC:2.3.2.27, E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B

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Galerie de données de validation

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  WB analysis of HEK-293 using 15621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15621-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15621-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15621-1-AP cible RNF40 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF40 Protéine recombinante Ag8047
• Nom complet: ring finger protein 40
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 110-140 kDa
• Numéro d’acquisition GenBank: BC006133
• Symbole du gène: RNF40
• Identification du gène (NCBI): 9810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolett (Verified Customer) (09-25-2025)

Antibody works well for Western Blotting after overnight incubation in 3% BSA/PBS and also in milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Lung Cancer Cell Lines

FH

Iram (Verified Customer) (03-11-2022)

This antibody works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Colon cancer cells

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