Anticorps Recombinant de lapin anti-RNF40

RNF40 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241214A1

N° de cat : 84058-5-PBS

Synonymes

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

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Galerie de données de validation

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  WB analysis using 84058-5-RR (same clone as 84058-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84058-5-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84058-5-RR (same clone as 84058-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF40 antibody (84058-5-RR, Clone: 241214A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84058-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84058-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84058-5-RR against Human RNF40 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84058-5-PBS cible RNF40 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RNF40 Protéine recombinante Ag8047
• Nom complet: ring finger protein 40
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC006133
• Symbole du gène: RNF40
• Identification du gène (NCBI): 9810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

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