Anticorps Polyclonal de lapin anti-RNF6

• Phare
• Validé par KD/KO

RNF6 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20437-1-AP

Synonymes

RNF6, SPG2

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Galerie de données de validation

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  WB analysis of mouse testis using 20437-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20437-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20437-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 20437-1-AP

  IP Result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 20437-1-AP

  Immunofluorescent analysis of HepG2 cells, using RNF6 antibody 20437-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules PC-3
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20437-1-AP cible RNF6 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF6 Protéine recombinante Ag14209
• Nom complet: ring finger protein (C3H2C3 type) 6
• Masse moléculaire calculée: 685 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC034688
• Symbole du gène: RNF6
• Identification du gène (NCBI): 6049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

Publications

Species	Application	Title
human,mouse	WB,IF,ChIP	Oncogene; RING-finger protein 6 promotes colorectal tumorigenesis by transcriptionally activating SF3B2.; Authors - Hui Xu ; KO Validated
human	IHC	Cancer Lett; Ring finger protein 6 enhances chemo-resistance by transcriptionally activating proliferating cell nuclear antigen expression and attenuating DNA damage in lung adenocarcinoma.; Authors - Yile Sun
human	WB	Acta Pharmacol Sin; RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.; Authors - Ying Ren
human	WB	Anticancer Drugs; miR-203a suppresses cell proliferation by targeting RING-finger protein 6 in colorectal cancer.; Authors - Jiyu Miao
human	WB,IP	J Biol Chem; Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia; Authors - Haixia Zhuang
human	WB,IHC	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun