Anticorps Polyclonal de lapin anti-RNF6

• Phare
• Validé par KD/KO

RNF6 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20437-1-PBS

Synonymes

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

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Galerie de données de validation

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  WB analysis using 20437-1-AP (same clone as 20437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

  IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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  IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20437-1-PBS cible RNF6 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF6 Protéine recombinante Ag14209
• Nom complet: ring finger protein (C3H2C3 type) 6
• Masse moléculaire calculée: 685 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC034688
• Symbole du gène: RNF6
• Identification du gène (NCBI): 6049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

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