Anticorps Polyclonal de lapin anti-RNF8

• Phare
• Validé par KD/KO

RNF8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(23)

N° de cat : 14112-1-AP

Synonymes

RING-type E3 ubiquitin transferase RNF8, RING finger protein 8, KIAA0646, hRNF8, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of HeLa using 14112-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14112-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14112-1-AP

  IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug.

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  IHC staining of human testis using 14112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14112-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF8 antibody (14112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

14112-1-AP cible RNF8 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF8 Protéine recombinante Ag5292
• Nom complet: ring finger protein 8
• Masse moléculaire calculée: 485 aa, 56 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: BC007517
• Symbole du gène: RNF8
• Identification du gène (NCBI): 9025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

Publications

Species	Application	Title
mouse	IF,WB	Cell; Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.; Authors - Lan-Tao Gou
human	IHC	Cell Death Differ; CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma; Authors - You Heng Chuah
human	IP, IF	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human	WB	EMBO J; The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.; Authors - Abhay Narayan Singh ; KD Validated
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB,IF	EMBO J; BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.; Authors - Zhaohua Hu

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