Anticorps Monoclonal anti-RNF8

RNF8 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G3H5

N° de cat : 68521-1-PBS

Synonymes

FLJ12013, KIAA0646, ring finger protein 8, RNF8

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Galerie de données de validation

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  WB analysis using 68521-1-Ig (same clone as 68521-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68521-1-Ig (RNF8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68521-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68521-1-PBS cible RNF8 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RNF8 Protéine recombinante Ag5292
• Nom complet: ring finger protein 8
• Masse moléculaire calculée: 485 aa, 56 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC007517
• Symbole du gène: RNF8
• Identification du gène (NCBI): 9025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

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