Anticorps Polyclonal de lapin anti-RNMT

• Phare
• Validé par KD/KO

RNMT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 13743-1-AP

Synonymes

hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, MET, mRNA cap methyltransferase, RG7MT1, RNMT

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Galerie de données de validation

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  WB analysis of human kidney using 13743-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13743-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13743-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13743-1-AP (RNMT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 13743-1-AP

  IP result of anti-RNMT(IP:13743-1-AP, 4ug; Detection:13743-1-AP 1:3000) with L02 cells lysate 1040 ug.

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  IHC staining of human colon cancer using 13743-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13743-1-AP (RNMT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 13743-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13743-1-AP (RNMT antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13743-1-AP

  Immunofluorescent analysis of Hela cells, using RNMT antibody 13743-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules HeLa, tissu hépatique humain
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13743-1-AP cible RNMT dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNMT Protéine recombinante Ag4682
• Nom complet: RNA (guanine-7-) methyltransferase
• Masse moléculaire calculée: 476 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036798
• Symbole du gène: RNMT
• Identification du gène (NCBI): 8731
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.

Publications

Species	Application	Title
human	WB	Cell; RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.; Authors - Fustin Jean-Michel JM ; KD Validated
human	WB	Nucleic Acids Res; Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.; Authors - Daniel Del Valle Morales
human	WB,CoIP	J Mol Biol; Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.; Authors - Michael J Osborne

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Karthik Subramanian (Verified Customer) (07-18-2019)

The antibody initially worked very well for my western blot applications. Subsequently, however, its performance has considerably become poorer and often results in fainter bands with some amount of non-specificity.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa