Anticorps Polyclonal de lapin anti-ROCK1

• Phare
• Validé par KD/KO

ROCK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21850-1-PBS

Synonymes

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1

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Galerie de données de validation

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  WB analysis using 21850-1-AP (same clone as 21850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21850-1-PBS cible ROCK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROCK1 Protéine recombinante Ag16317
• Nom complet: Rho-associated, coiled-coil containing protein kinase 1
• Masse moléculaire calculée: 1354 aa, 158 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC113114
• Symbole du gène: ROCK1
• Identification du gène (NCBI): 6093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

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