Anticorps Monoclonal anti-ROCK2

• Phare
• Validé par KD/KO

ROCK2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C7B8

N° de cat : 66633-1-PBS

Synonymes

1C7B8, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2

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Galerie de données de validation

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  WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

  WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66633-1-PBS cible ROCK2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ROCK2 Protéine recombinante Ag16330
• Nom complet: Rho-associated, coiled-coil containing protein kinase 2
• Masse moléculaire calculée: 77 kDa, 161 kDa
• Poids moléculaire observé: 161 kDa
• Numéro d’acquisition GenBank: BC111801
• Symbole du gène: ROCK2
• Identification du gène (NCBI): 9475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

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